About Preprint OnlineArticles prepublished May 25, 2012
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Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished May 03, 2012
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Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished April 27, 2012
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Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished April 26, 2012
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Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished April 04, 2012
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Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished April 03, 2012
|
|
Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished March 22, 2012
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|
Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished March 08, 2012
|
|
Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished February 28, 2012
|
|
Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
Articles prepublished December 21, 2011
|
|
Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus
H. Hu (1), L. Zhu (1, 2), Z. Huang (1), Q. Ji (3), M. Chatterjee (2), W. Zhang (2), N. Li (1, 2)
(1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China; (2) Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden; (3) Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Summary
Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
Keywords
thrombosis, lymphocytes, Platelets, platelet-lymphocyte conjugates, arterial flow
DOI
http://dx.doi.org/10.1160/TH10-05-0308You may also be interested in...
| 1. | ||
Ok-Nam Bae*1, Young-Dae Kim*1, Kyung-Min Lim1,2, Ji-Yoon Noh1, Seung-Min Chung1, Keunyoung Kim1, Suyoung Hong1, Sue Shin3, Jong-Hyun Yoon3, Jin-Ho Chung1 Thrombosis and Haemostasis 2008 100 1: 52-59 http://dx.doi.org/10.1160/TH07-08-0529 | ||
| 2. | ||
Comparison of PD0348292, a selective factor Xa inhibitor, to antiplatelet agents for the inhibition of arterial thrombosis Krzysztof Karnicki2, Robert J. Leadley Jr. 3, Sangita Baxi3, Thomas Peterson3,Waldemar Wysokinski1,2, Robert D. McBane, II1,2 Thrombosis and Haemostasis 2008 99 4: 759-766 http://dx.doi.org/10.1160/TH07-09-0576 | ||
| 3. | ||
A. J. Reininger Hämostaseologie 2007 27 4: 247-250 | ||
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